Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat
- IMMUNOGEN: The immunogen for this antibody is: C-KVYSKEDQDLLK
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Human Lymph Node and Human Spleen lysates (calculated MW of 25.3kDa according to NP_031386.2). The observed molecular weight is explained by glycosylation (Han et al, Blood. 2004 Nov 1;104(9):2858-66. Epub 2004 Jul 6; PMID: 15238421). Recommended concentration: 0.1-0.3ug/ml. Primary incubated for 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Spleen, and Rat Thymus for this particular batch.
- POSITIVE CONTROL 1: Cat. No. 1306 - Human Spleen Tissue Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: KLRK1
- ADDITIONAL NAMES: KLRK1, NKG2-D, killer cell lectin-like receptor subfamily K, member 1, HGNC:18788, D12S2489E, KLR, NK cell receptor D, NKG2-D type II integral membrane protein, CD314, D12S2489E, FLJ17759, FLJ75772, NKG2D
- Protein Accession Number: NP_031386.2
- PROTEIN GI NUMBER: 6679052
- NCBI GENE ID NUMBER: 22914
Background
- 1: Hayakawa Y, Smyth MJ. Innate immune recognition and suppression of tumors. Adv Cancer Res. 2006;95:293-322
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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