KIT / CD117 Antibody
CATALOG NUMBER: 43-381- Clonality: Polyclonal
- Tested Applications: E, Flow, IF, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
- IMMUNOGEN: The immunogen for this antibody is: KRDSFICSKQEDH
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, Flow, IF, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
- SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_000213.1; NP_001087241.1).
- PREDICTED MOLECULAR WEIGHT: Approx 110kDa
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: KIT / CD117
- ADDITIONAL NAMES: KIT, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit, CD117, PBT, SCFR, mast/stem cell growth factor receptor Kit, p145 c-kit, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, soluble KIT varian
- Protein Accession Number: NP_000213.1,NP_001087241.1
- PROTEIN GI NUMBER: 125472
- NCBI GENE ID NUMBER: 3815
Background
- 1: Herpel E, Jensen K, Muley T, Warth A, Schnabel PA, Meister M, Herth FJ, Dienemann H, Thomas M, Gottschling S.The cancer stem cell antigens CD133, BCRP1/ABCG2 and CD117/c-KIT are not associated with prognosis in resected early-stage non-small cell lung cancer.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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