- Trial Size
KIRREL2 Antibody
CATALOG NUMBER: 7971- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
Powered by BiozSpecifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: KIRREL2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KIRREL2.
The immunogen is located within amino acids 550 - 600 of KIRREL2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: KIRREL2 antibody can be used for detection of KIRREL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: KIRREL2 antibody is human, mouse and rat reactive. At least five isoforms are are known to exist. This antibody is predicted to not cross-react with other members of the KIRREL protein family.
- POSITIVE CONTROL 1: Cat. No. 1210 - HEK293 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 11-101 - Human Pancreas Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 64, 70, 78 kDa
Observed: 70 kDa
Properties
- PURIFICATION: KIRREL2 antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: KIRREL2 antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: KIRREL2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: KIRREL2
- ADDITIONAL NAMES: Kin of irregular chiasm-like protein 2, Kin of IRRE-like protein 2, NEPH3, Nephrin-like protein 3, NLG1, FILTRIN
- Protein Accession Number: NP_954649
- PROTEIN GI NUMBER: 145275174
- NCBI GENE ID NUMBER: 84063
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- KIRREL2, also known as nephrin-like 3, is a type I transmembrane protein belonging to the immunoglobulin superfamily of cell adhesion molecules (1). KIRREL2 consists of five extracellular Ig-like repeats, a transmembrane domain, several glycosylation sites and a cytoplasmic domain that has a stretch of nine conserved residues (1,2). KIRREL2 has been found to be expressed in beta cells of pancreatic islets and is involved in the regulation of both glomerular and neural development (3,4). Downregulation of KIRREL2 is implicated in several proteinuric diseases (4,5).
- 1: Sun C, Kilburn D, Lukashin A, et al. KIRREL2, a novel immunoglobulin superfamily gene expressed primarily in beta cells of the pancreatic islets. Genomics 2003; 82:130-42.
- 2: Heikkila E, Ristola M, Havana M, et al. Trans-interaction of nephrin and Neph1/Neph3 induces cell adhesion that associates with decreased tyrosine phosphorylation of nephrin. Biochem. J. 2011; 435:619-28.
- 3: Wang H, Lehtonen S, Chen YC, et al. Neph3 associates with regulation of glomerular and neural development in zebrafish. Differentiation 2012; 83:38-46.
- 4: Mizuhara E, Minaki Y, Nakatani T, et al. Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin. Dev. Biol. 2010; 338:202-14.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
New & Featured Products
![HIV-1 p24 Antibody [10A2] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-10A2_WB_PM-7371.gif){kind=small}
HIV-1 p24 Antibody [10A2] (biotin)
CATALOG NUMBER: PM-7371-biotin
![HIV-1 p24 Antibody [9D2F6] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-9D2F6_WB_PM-7369.gif){kind=small}
HIV-1 p24 Antibody [9D2F6] (biotin)
CATALOG NUMBER: PM-7369-biotin
![HIV-1 p24 Antibody [8D7E11] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-8D7E11_WB_PM-7367.gif){kind=small}
HIV-1 p24 Antibody [8D7E11] (biotin)
CATALOG NUMBER: PM-7367-biotin