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Home / Flow / Kappa Light Chain Antibody [KLC709]
Kappa Light Chain Antibody [KLC709]Kappa Light Chain Antibody [KLC709]
Kappa Light Chain Antibody [KLC709]
Kappa Light Chain Antibody [KLC709]

Kappa Light Chain Antibody [KLC709]

CATALOG NUMBER: 33-151
  • Clonality: Monoclonal
  • Tested Applications: Flow, IHC, WB
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Recombinant human Kappa light chain was used as the immunogen for this anti-Kappa light chain antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, IHC, WB
  • APPLICATION NOTE: Western blot: 0.5-1 ug/ml
    Flow Cytometry: 0.5-1 ug/million cells
    IHC (Frozen
    FFPE): 0.5-1.0 ug/ml for 30 min at RT (1)
    Prediluted format : incubate for 30 min at RT (2)
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-Kappa light chain antibody to be titered up or down for optimal performance.

    1. Staining of FFPE tissues requires boiling sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: IGKC
  • NCBI GENE ID NUMBER: 3514
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-151

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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