- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (86%), Pig: (79%)
- IMMUNOGEN: IRF3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF3.
The immunogen is located within amino acids 330 - 380 of IRF3.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
- SPECIFICITY: At least three isoforms of IRF3 are known to exist.
- POSITIVE CONTROL 1: Cat. No. 1225 - Ramos Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1405 - Mouse Kidney Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: 43, 47, 50 kDa
- PURIFICATION: IRF3 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: IRF3 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: IRF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: IRF3
- ADDITIONAL NAMES: IRF3 Antibody: Interferon regulatory factor 3, IRF-3
- Protein Accession Number: NP_001562
- PROTEIN GI NUMBER: 4504725
- NCBI GENE ID NUMBER: 3661
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- IRF3 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.
- 1: Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
- 2: Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.
- 3: Fitzgerald KA, McWhirter SM, Faia KL, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat. Immunol. 2003; 4:491-6.
- 4: Sharma S, Tenoever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 3397
- Size: 0.02 mg | 0.1 mg
- List Price: $109.00 – $379.00
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