- Trial Size
IRAK2 Antibody
CATALOG NUMBER: 3595- Clonality: Polyclonal
- Tested Applications: E, ICC, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (88%), Bovine: (82%)
- IMMUNOGEN: IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a.
The immunogen is located within the last 50 amino acids of IRAK2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, ICC, IF, WB
- APPLICATION NOTE: IRAK2 antibody can be used for detection of IRAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. - SPECIFICITY: At least four isoforms of IRAK2 are known to exist; this antibody will detect all four isoforms. Anti-IRAK2 has no cross response to IRAK.
- POSITIVE CONTROL 1: Cat. No. 1283 - RAW 264.7 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1288 - A20 Cell Lysate
- POSITIVE CONTROL 3: Cat. No. 17-208 - A-20 Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 68 kDa
Observed: 65 kDa
Properties
- PURIFICATION: IRAK2 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: IRAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: IRAK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: Irak2
- ADDITIONAL NAMES: IRAK2 Antibody: IRAK-2, AI649099, 6330415L08Rik, Interleukin-1 receptor-associated kinase-like 2, IRAK-2
- Protein Accession Number: AAO24761
- PROTEIN GI NUMBER: 37725373
- NCBI GENE ID NUMBER: 108960
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- IRAK2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.
- 1: Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
- 2: Zhang FX, Kirschning CJ, Mancinelli R, et al. Bacterial lipopolysaccharide activates nuclear factor-κB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J. Biol. Chem. 1999; 274:7611-4.
- 3: Yang RB, Mark MR, Gurney AL, et al. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J. Immunol. 1999; 163:639-43.
- 4: Hardy MP and O'Neill LAJ. The murine IRAK2 gene encodes four alternately spliced isoforms, two of which are inhibitory. J. Biol. Chem. 2004; 279:27699-708.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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