- Trial Size
INPP4B Antibody
CATALOG NUMBER: 8339- Clonality: Polyclonal
- Tested Applications: ELISA, ICC, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: INPP4B antibody was raised against a 17 amino acid peptide near the amino terminus of human INPP4B.
The immunogen is located within the last 50 amino acids of INPP4B. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, ICC, WB
- APPLICATION NOTE: INPP4B antibody can be used for detection of INPP4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. - SPECIFICITY: INPP4B antibody is human, mouse and rat reactive. At least two isoforms of INPP4B are known to exist; this antibody only detects the longer isoform. INPP4B antibody is predicted to not cross-react with INPP4A.
- POSITIVE CONTROL 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-201 - 3T3/BALB Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 102 kDa
Observed: 100 kDa
Properties
- PURIFICATION: INPP4B antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: INPP4B antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: INPP4B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: INPP4B
- ADDITIONAL NAMES: Inositol polyphosphate-4-phosphatase type II 105kDa
- Protein Accession Number: NP_003857
- PROTEIN GI NUMBER: 156104895
- NCBI GENE ID NUMBER: 8821
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Inositol polyphosphate 4-phosphatase type II (INPP4B) is one of the enzymes involved in phosphatidylinositol signaling pathways. It removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate (1). INPP4B can function as a tumor suppressor by inhibiting PI3K signaling. In human epithelial cells, knockdown of INPP4B expression resulted in enhanced Akt activation, anchorage-independent growth, and enhanced overall motility (2). It is thought INPP4B may also have oncogenic functions as INPP4B-dependent PI3K signaling is mediated by SGK3 in breast cancer cells and expression of INPP4B leads to enhanced SGK3 activation and suppression of AKT phosphorylation (3).
- 1: Norris FA, Auethavekiat V, and Majerus PW. The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphase. J. Biol. Chem. 1995; 270:16128-33.
- 2: Gewinner C, Wang ZC, Richardson A, et al. Evidence that inositol polyphosphate 4-phosphase type II is a tumor suppressor that inhibits PI3K signaling. Cancer Cell 2009; 16:115-25.
- 3: Gasser JA, Inuzuka H, Lau AW, et al. SGK3 mediates INPP4B-dependent PI3K signaling in breast cancer. Mol. Cell 2014; 56:595-607.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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