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IL-9 Antibody
IL-9 Antibody
IL-9 Antibody
IL-9 Antibody
IL-9 Antibody
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IL-9 Antibody

CATALOG NUMBER: 7495
  • Clonality: Polyclonal
  • Tested Applications: E, IF, IHC-P, WB
  • Host Species: Rabbit
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Rabbit
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Anti-IL-9 antibody (7495) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IL-9.
    The immunogen is located within the last 50 amino acids of IL-9.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: E, IF, IHC-P, WB
  • APPLICATION NOTE: WB: 1-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
    Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
  • SPECIFICITY: IL-9 antibody is human specific. IL-9 antibody is predicted to no cross-react with IL-7.
  • POSITIVE CONTROL 1: Cat. No. 1306 - Human Spleen Tissue Lysate
  • POSITIVE CONTROL 2: Cat. No. 10-901 - Human Spleen Tissue Slide
  • POSITIVE CONTROL 3: Cat. No. 1201 - HeLa Cell Lysate
  • PREDICTED MOLECULAR WEIGHT: Predicted: 16kD
    Observed: 22 kD (Post-modification: 4 N-linked glycosylations)

Advanced Validation

  • VALIDATION:

    Recombinant Protein Test (Figure 2): Anti-IL-9 antibodies (7495) detected human IL-9 recombinant protein at different concentrations.

  • ISOFORMS: Human IL-9 has one isoform (144aa, 16kD). Mouse IL-9 has one isoform (144aa, 16kD) and Rat IL-9 also has one isoform (144aa, 16kD). 7495 can detect human isoform, but cannot detect mouse and rat.

Properties

  • PURIFICATION: IL-9 Antibody is affinity chromatography purified via peptide column.
  • CLONALITY: Polyclonal
  • ISOTYPE: IgG
  • PHYSICAL STATE: Liquid
  • BUFFER: IL-9 Antibody is supplied in PBS containing 0.02% sodium azide.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: IL-9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Additional Info

  • NCBI OFFICIAL SYMBOL: IL9
  • ADDITIONAL NAMES: IL-9 Antibody : P40, HP40, IL-9, Interleukin-9, Cytokine P40
  • Protein Accession Number: NP_000581
  • PROTEIN GI NUMBER: 10834980
  • NCBI GENE ID NUMBER: 3578
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • Interleukin 9 (IL-9) is a cytokine secreted by TH2 lymphocytes that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation and prevents apoptosis (1,2). It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins (3). The IL-9 gene has been identified as a candidate gene for asthma (4). Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness (5).
  • 1: Renauld et al. Cytokine 1990; 2:9-12.
  • 2: Tete et al. J. Biol. Homeost. Agents 2012; 26:319-26.
  • 3: Knoops and Renauld. Growth Factors 2004; 22:207-15.
  • 4: Nicolaides et al. Proc. Natl. Acad. Sci. USA 1997; 94:13175-80.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 7495

  • Size: 0.02 mg | 0.1 mg
  • List Price: $89.00 – $395.00
Datasheet
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