IL-9 Antibody
CATALOG NUMBER: XP-5200- Clonality: Polyclonal
- Tested Applications: ELISA, Neutralization Assay, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-9 (human Interleukin-9).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Neutralization Assay, WB
- APPLICATION NOTE: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-9 (0.6 ng/mL), a concentration of 0.15 - 0.025 μg/mL of this antibody is required.
ELISA:
To detect hIL-9 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-9.
Sandwich:
To detect hIL-9 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-9 (XP-5200Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-9.
Western Blot:
To detect hIL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Anti-hIL-9 specific antibody was purified by affinity chromatography employing immobilized hIL-9 matrix.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: IL-9 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: IL9
- ADDITIONAL NAMES: P40, HP40, IL-9Interleukin-9, Cytokine P40, IL-9
- Protein Accession Number: P15248
- PROTEIN GI NUMBER: 124362
- NCBI GENE ID NUMBER: 3578
- USER NOTE: Centrifuge vial prior to opening.
Background
- IL-9 is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors; but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL-9 stimulates cell proliferation and prevents apoptosis. The IL-9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL-9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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