IL-6 Antibody
CATALOG NUMBER: XP-5192- Clonality: Polyclonal
- Tested Applications: ELISA, Neutralization Assay, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-6 (human Interleukin-6).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Neutralization Assay, WB
- APPLICATION NOTE: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (1.0 ng/mL), a concentration of 0.2 -0.4 μg/mL of this antibody is required.
ELISA:
To detect hIL-6 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-6.
Sandwich:
To detect hIL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-6 (XP-5192Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6.
Western Blot:
To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Anti-hIL-6 specific antibody was purified by affinity chromatography employing immobilized hIL-6 matrix.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: IL-6 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: IL6
- ADDITIONAL NAMES: HGF, HSF, BSF2, IL-6, IFNB2, Interleukin-6, B-cell stimulatory factor 2
- Protein Accession Number: P05231
- PROTEIN GI NUMBER: 124347
- NCBI GENE ID NUMBER: 3569
- USER NOTE: Centrifuge vial prior to opening.
Background
- IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6 appears to interact with IL2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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