IL-33 Recombinant Protein
CATALOG NUMBER: 95-111- Tested Applications: ELISA, WB
- Size: 0.05 mg
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- SPECIES: Human
- SOURCE SPECIES: E. coli
- RECOMBINANT PROTEIN SEQUENCE: aa 112 - 270
- FUSION TAG: Fusion Partner: C-terminal His-tag
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: This recombinant protein can be used for WB and ELISA. For research use only.
- PREDICTED MOLECULAR WEIGHT: 22 kDa (Calculated)
Properties
- PURITY: ~95%
- PHYSICAL STATE: Liquid
- BUFFER: 100mM sodium phosphate, 500mM NaCl, 25 mM imidazole, 10% gycerol
- STORAGE CONDITIONS: Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Additional Info
- NCBI OFFICIAL SYMBOL: IL33
- ADDITIONAL NAMES: IL-33 Antibody: DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33
- Protein Accession Number: NP_254274
- PROTEIN GI NUMBER: 15559209
- NCBI GENE ID NUMBER: 90865
Background
- Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1, IL-1Ra and IL-18 (1,2). Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling (1,3) and IL-1R accessory protein (IL-1RAcP) (4). Receptor binding of IL-33 activates NF-B and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine (2). Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties (5). This recombinant protein represents the cleaved and presumably activated form of IL-33, but has not been tested in any biological assays.
- 1: Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 2005; 23:479-90.
- 2: Dinarello CA. Interleukin-18, a proinflammatory cytokine. Eur. Cytokine Netw. 2000; 11:483-6.
- 3: Brint EK, Xu D, Liu H, et al. ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance. Nat. Immunol. 2004; 5:373-9.
- 4: Chackerian AA, Oldham ER, Murphy EE, et al. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol. 2007; 179:2551-5.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
Shipping Info
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