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IL-33 Antibody
CATALOG NUMBER: 4273- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (76.5%)
- IMMUNOGEN: Anti-IL-33 antibody (4273) was raised against a 17 amino acid synthetic peptide from near the center of human IL-33.
The immunogen is located within the 150-200 amino acids of IL-33. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: WB: 1-2 μg/mL; IF: 20 μg/mL; IHC-P: 2.5 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1288 - A20 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 31kD
Observed: 33kD
Advanced Validation
- VALIDATION:
Recombinant Protein Validation (Figure 2) shows expression of human partial recombinant protein IL-33 detected by anti-IL-33 antibody, 4273. Bands of 18kD (monomer) and 36kD (dimer) were observed.
Regulated Expression Validation (Figure 7&8) shows the up-regulation (Figure 7) of IL-33 detected by anti-IL-33 antibody in human skin fibroblasts after α-irradiation and this effect was inhibited by PPP (Figure 8).
- ISOFORMS: Human IL-33 has 4 isoforms, including isoform 1 (270aa, 30.8kD), isoform 2 (228aa, 25.9kD), isoform 3 (228aa, 26.1kD) and isoform 4 (144aa, 16.3kD). This antibody detects only human isoform L. Mouse IL-33 has 1 isoform (266aa, 30kD). Rat IL-33 has only one isoform identified so far (264aa, 29.5kD).
Properties
- PURIFICATION: IL-33 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: IL-33 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: IL-33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: IL33
- ADDITIONAL NAMES: IL-33 Antibody: DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33
- Protein Accession Number: NP_254274
- PROTEIN GI NUMBER: 15559209
- NCBI GENE ID NUMBER: 90865
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- IL-33 Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1α/beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
- 1: Schmitz J et al. Immunity 2005; 23:479-90.
- 2: Dinarello Eur. Cytokine Netw. 2000; 11:483-6.
- 3: Brint et al. Nat. Immunol. 2004; 5:373-9.
- 4: Chackerian et al. J. Immunol. 2007; 179:2551-5.
Citations
- 1: Ivanov et al. Radiation-induced bystander signaling pathways in human fibroblasts: a role for interleukin-33 in the signal transmission.Cell Signal. 2010 Jul;22(7):1076-87. PMID: 20206688
- 2: Tsuda et al. Novel splice variants of IL-33: differential expression in normal and transformed cells. J Invest Dermatol. 2012 Nov;132(11):2661-4. PMID: 22785132
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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