IL-3 beta Antibody (biotin)
CATALOG NUMBER: 38-343- Clonality: Polyclonal
- Tested Applications: E, WB
- Host Species: Goat
- Species Reactivity: Rat
- Conjugate: Biotin
- Size: 0.025 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Rat
- IMMUNOGEN: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-3beta. Anti-Rat IL-3beta specific antibody was purified by affinity chromatography and then biotinylated.
- CONJUGATE: Biotin
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: Sandwich ELISA: To detect Rat IL-3 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Rat IL-3 beta as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-3 beta.
Western Blot
To detect Rat IL-3 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: IL-3 beta specific antibody was purified by affinity chromatography and then biotinylated
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: Il3
- ADDITIONAL NAMES: MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor
- Protein Accession Number: P97688
- PROTEIN GI NUMBER: P04823.1
- NCBI GENE ID NUMBER: 24495
- USER NOTE: Centrifuge vial prior to opening.
Background
- IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine. Recombinant Rat IL-3beta is a 16.3 kDa globular protein containing 144 amino acid residues.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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