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IL-21 Antibody
CATALOG NUMBER: 2465- Clonality: Polyclonal
- Tested Applications: ELISA, ICC, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (87%)
- IMMUNOGEN: Anti-IL-21 antibody (2465) was raised against a peptide corresponding to 15 amino acids near the center of human IL-21 precursor.
The immunogen is located within the last 50 amino acids of IL-21. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, ICC, WB
- APPLICATION NOTE: WB: 1 μg/mL; ICC: 2 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested. - SPECIFICITY: At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.
- POSITIVE CONTROL 1: Cat. No. 1209 - HL60 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-004 - K-562 Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 18kD
Observed: 17 kD
Advanced Validation
- VALIDATION:
Independent Antibody Validation in Cell Lines (Figure 2) shows similar IL-21 expression profile in human and mouse cell lines detected by two independent anti-IL-21 antibodies that recognize different epitopes, 2465 against central domain and 2463 against C-terminus domain. IL-21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
Recombinant Protein Test (Figure 4): Anit-IL-21 antibodies (2465) detected human IL-21 recombinant protein.
Regulated expression validation (Figure 6, 7, 8): IL-21 expression detected by anit-IL-21 antibodies (2465) was up down-regulated by WRT treatment (figure 7) or was up-regulated in Hp positive patients (figure 8) and in patients with Crohn’s disease (figure 6).
- ISOFORMS: Human IL-21 has 2 isoforms, including isoform 1 (162aa, 18.7kD) and isoform 2 (153aa, 17.6kD). Mouse IL-21 has one isoform (146aa, 16.8kD) and Rat IL-21 also has one isoform (146aa, 17.1kD). 2465 can detect both human isoforms and also detect mouse and rat.
Properties
- PURIFICATION: IL-21 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: IL-21 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/ml
- STORAGE CONDITIONS: IL-21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: IL21
- ADDITIONAL NAMES: IL-21 Antibody: Za11, IL-21, Interleukin-21, Za11
- Protein Accession Number: NP_068575
- PROTEIN GI NUMBER: 11141875
- NCBI GENE ID NUMBER: 59067
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- IL-21 Antibody: A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
- 1: Parrish-Novak et al. Nature 2000; 408:57-63.
- 2: Ozaki et al. Proc. Natl. Acad. Sci. USA. 2000; 97:11439-44.
- 3: Asao et al. J. Immunol. 2001; 167:1-5.
Citations
- 1: Monteleone et al. Interleukin-21 enhances T-helper cell type I signaling and interferon-γ production in Crohn's disease. Gastroenterology. 2005;128(3):687-94. PMID: 15765404
- 2: Sarra et al. IL-15 positively regulates IL-21 production in celiac disease mucosa. Mucosal Immunol. 2013;6(2):244-55. PMID: 22785229
- 3: Caruso et al. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis. J Immunol. 2007;178(9):5957-65. PMID: 17442980
- 4: Daniele Fina. Interleukin-21 controls inflammatory pathways in IBD. University of Rome, Italy. PhD thesis. 2009.PMID:
- 5: Roberta Caruso. Expression and functional role of Th-17 cells in helicobacter pylori-related gastritis. University of Rome, Italy. PhD thesis. 2008.PMID:
- 6: Giovanni Monteleone. Antigenicepitopes of Interleukin-21, related antibodies and their use in medical field. U.S patent application publication. 2009.PMID:
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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