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IL-2 Antibody [5B9-2-1]IL-2 Antibody [5B9-2-1]IL-2 Antibody [5B9-2-1]
IL-2 Antibody [5B9-2-1]
IL-2 Antibody [5B9-2-1]
IL-2 Antibody [5B9-2-1]

IL-2 Antibody [5B9-2-1]

CATALOG NUMBER: 38-299
  • Clonality: Monoclonal
  • Tested Applications: ELISA, Neutralization Assay, WB
  • Host Species: Rat
  • Species Reactivity: Mouse
  • Conjugate: Unconjugated
  • Size: 0.5 mg

Specifications

  • HOST SPECIES: Rat
  • SPECIES REACTIVITY: Mouse
  • IMMUNOGEN: E.coli derived Recombinant Murine IL-2
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: ELISA, Neutralization Assay, WB
  • APPLICATION NOTE: ELISA:
    Sandwich:
    In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL.
    Western Blot:
    To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μg/mL of this antibody is required.

Properties

  • PURIFICATION: This IgG2Ak antibody was purified from cell culture by Protein A affinity chromatography.
  • CLONALITY: Monoclonal
  • PHYSICAL STATE: Lyophilized
  • CONCENTRATION: batch dependent
  • STORAGE CONDITIONS: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: IL2
  • ADDITIONAL NAMES: Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine
  • Protein Accession Number: P60568
  • PROTEIN GI NUMBER: 45593462
  • NCBI GENE ID NUMBER: 3558
  • USER NOTE: Centrifuge vial prior to opening.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: This product is for research use only.

CATALOG NUMBER: 38-299

  • Size: 0.5 mg
  • List Price: $255.00
Datasheet
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