IL-13 Antibody (biotin)
CATALOG NUMBER: 38-345- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Rabbit
- Species Reactivity: Rat
- Conjugate: Biotin
- Size: 0.025 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Rat
- IMMUNOGEN: Produced from sera of rabbits immunized with highly pure recombinant Rat IL-13. Anti-Rat IL-13-specific antibody was purified by affinity chromatography and then biotinylated.
- CONJUGATE: Biotin
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: Sandwich ELISA: To detect Rat IL-13 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti- Rat IL-13 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-13.
Western Blot
To detect Rat IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat IL-13 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: IL-13 specific antibody was purified by affinity chromatography and then biotinylated
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: Il13
- ADDITIONAL NAMES: NC300 (Human), P600 (Murine)
- Protein Accession Number: P42203
- PROTEIN GI NUMBER: P42203.1
- NCBI GENE ID NUMBER: 116553
- USER NOTE: Centrifuge vial prior to opening.
Background
- IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1b, TNF-a, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 are cross-species reactive. Recombinant Rat IL-13 a 12.3 kDa protein consisting of 113 amino acid residues.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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