IL-10 Antibody
CATALOG NUMBER: XP-5163- Clonality: Polyclonal
- Tested Applications: ELISA, Neutralization Assay, WB
- Host Species: Rabbit
- Species Reactivity: Rat
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Rat
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rIL-10 (rat Interleukin-10).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Neutralization Assay, WB
- APPLICATION NOTE: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of rIL-10 (30.00 ng/mL), a concentration of 0.45 0.8 mg/mL of this antibody is required.
ELISA:
To detect rIL-10 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rIL-10.
Sandwich
To detect Rat IL-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-10 (XP-5163Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.
Western Blot:
To detect rIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Anti-rIL-10 specific antibody was purified by affinity chromatography employing immobilized rIL-10 matrix.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: IL-10 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: Il10
- ADDITIONAL NAMES: IL10X, Il-10, Interleukin-10, Cytokine synthesis inhibitory factor, IL-10
- Protein Accession Number: P29456
- PROTEIN GI NUMBER: 266338
- NCBI GENE ID NUMBER: 25325
- USER NOTE: Centrifuge vial prior to opening.
Background
- Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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