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Specifications
- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Rat
- IMMUNOGEN: E.coli derived Recombinant Rat IL-10
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 8.0-16.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant Rat IL-10 when used with ProSci’s Polyclonal Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Properties
- PURIFICATION: This antibody was purified from tissue culture by Protein A affinity chromatography.
- CLONALITY: Monoclonal
- PHYSICAL STATE: Lyophilized
- STORAGE CONDITIONS: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: IL10
- ADDITIONAL NAMES: Interleukin 10Interleukin-10, Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A
- Protein Accession Number: NP_000563.1
- PROTEIN GI NUMBER: 10835141
- NCBI GENE ID NUMBER: 3586
- USER NOTE: Centrifuge vial prior to opening.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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