IKK a (phospho Thr23) Antibody
CATALOG NUMBER: 79-161- Clonality: Polyclonal
- Tested Applications: IHC, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: IKK a (Phospho-Thr23) antibody was raised against a peptide sequence around phosphorylation site of threonine 23 (L-G-T (p) -G-G) derived from Human IKK α.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC, WB
- APPLICATION NOTE: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
- SPECIFICITY: This antibody detects endogenous level of IKKα only when phosphorylated at threonine 23.
- PREDICTED MOLECULAR WEIGHT: 85 kDa
Properties
- PURIFICATION: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Store antibody at -20°C for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: CHUK
- ADDITIONAL NAMES: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, CTNNB, OK/SW-cl.35, PRO2286, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK1
- Protein Accession Number: NP_001269.3
- PROTEIN GI NUMBER: 62241001
- NCBI GENE ID NUMBER: 1147
Background
- Acts as part of the IKK complex in the conventional pathway of NF-κ-B activation and phosphorylates inhibitors of NF-κ-B thus leading to the dissociation of the inhibitor/NF-κ-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-κ-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-κ-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-κ-B-regulated promoters during inflammatory responses triggered by cytokines.
- 1: Yuan ZQ, et al. (2002) J Biol Chem; 277 (33): 29973-82. Ozes ON, et al. (1999) Nature; 401 (6748): 82-5.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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