Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
- IMMUNOGEN: The immunogen for this antibody is: C-LNQLFPGYGNGR
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 100kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 91.1kDa according to NP_037414.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Hopfner, Cancer Res. 2000 Jan 1;60(1):121-8. PMID: 10646863). Recommended concentration: 1-3ug/ml.
- SPECIFICITY: This antibody is expected to recognise both reported isoforms.
- PREDICTED MOLECULAR WEIGHT: Approx 100kDa
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: ICBP90 / UHRF1
- ADDITIONAL NAMES: UHRF1, ubiquitin-like, containing PHD and RING finger domains, 1, Np95, ICBP90, FLJ21925, transcription factor ICBP90, RNF106, MGC138707, RING finger protein 106, inverted CCAAT box-binding protein of 90 kDa, nuclear zinc finger protein Np95, transcription factor ICBP90, ubiquitin-like with PHD and ring finger domains 1, hNP95, E3 ubiquitin-protein ligase UHRF1,
- Protein Accession Number: NP_001041666.1,NP_037414.3
- PROTEIN GI NUMBER: 115430235
- NCBI GENE ID NUMBER: 29128
Background
- 1: Hopfner R, Mousli M, Jeltsch JM, Voulgaris A, Lutz Y, Marin C, Bellocq JP, Oudet P, Bronner C. ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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