ICAM1 Antibody
CATALOG NUMBER: 43-164- Clonality: Polyclonal
- Tested Applications: E, IF, WB
- Host Species: Goat
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: The immunogen for this antibody is: SNCPDGQSTAKT
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 100kDa band observed in lysates of cell lines Daudi, HeLa and NIH3T3 (calculated MW of 57.8kDa according to Human NP_000192.2 and 58.8kDa according to Mouse NP_034623.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pino et al, Reproduction. 2009 Nov;138(5):837-47. PMID: 19661147), and other commercial sources. Recommended concentration: 1-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membrane of NIH3T3 cells. Recommended concentration: 5ug/ml.
- SPECIFICITY: The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite.
- PREDICTED MOLECULAR WEIGHT: Approx 100kDa
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: ICAM1
- ADDITIONAL NAMES: ICAM1, intercellular adhesion molecule 1, BB2, CD54, P3.58, ICAM-1, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor
- Protein Accession Number: NP_000192.2
- PROTEIN GI NUMBER: 68067956
- NCBI GENE ID NUMBER: 3383
Background
- 1: Fu C, Tong C, Wang M, Gao Y, Zhang Y, Lü S, Liang S, Dong C, Long M.Determining beta2-integrin and intercellular adhesion molecule 1 binding kinetics in tumor cell adhesion to leukocytes and endothelial cells by a gas-driven micropipette assay.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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