I kappa B Kinase Detection Set
CATALOG NUMBER: PSI-1821- Clonality: Polyclonal
- Tested Applications: ICC, IF, IHC, WB
- Species Reactivity: Human
- Size: 1 Set
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- SPECIES REACTIVITY: Human
- IMMUNOGEN: Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
- TESTED APPLICATIONS: ICC, IF, IHC, WB
- SET CONTENTS: IKKα Antibody, Catalog No. 2025 (50 μg)
IKKβ Antibody, Catalog No. 2121 (50 μg)
IKKγ Antibody, Catalog No. 2335 (50 μg)
IKKε Antibody, Catalog No. 2329 (50 μg) - APPLICATION NOTE: These polyclonal antibodies can be used for detection of IKKα, IKKβ, IKKγ and IKKε by immunoblot at 0.5 - 2 μg/mL, and by immuncytochemistry (IKKα, IKKβ and IKKγ) or immunohistochemistry (IKKε) at 1 - 10 μg/mL, and Immunoflourescence.
- POSITIVE CONTROL 1: IKKα Antibody: HeLa Cell Lysate, Catalog No. 1201
IKKβ Antibody: Jurkat Cell Lysate, Catalog No. 1223
IKKγ Antibody: HeLa Cell Lysate, Catalog No. 1201
IKKε Antibody: Jurkat Cell Lysate, Catalog No. 1223
Properties
- PURIFICATION: Antibodies are supplied as affinity chromatography purified IgG.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide.
- CONCENTRATION: Antibody 1 mg/mL
- STORAGE CONDITIONS: Stable at 4°C for three months, store at -20°C for up to one year.
Additional Info
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB kinase (IKK), which phosphorylates IκB and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IκB-α, while IKKβ phosphorylates both IκB-α and IκB-β. Another molecule in the IKK complex termed IKKγ (also known as NEMO) interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. IKKγ was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation. IKKε is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.
For images please see PDF data sheet - 1: DiDonato JA, Hayakawa M, Rothwarf DM, et al. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 1997; 388:548-54.
- 2: Regnier CH, Song HY, Gao X, et al. Identification and characterization of an IkappaB kinase. Cell 1997; 90:373-83.
- 3: Zandi E, Rothwarf DM, Delhase M, et al. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell 1997; 91:243-52.
- 4: Woronicz JD, Gao X, Cao Z, et al. IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK. Science 1997; 278:866-9.
Set Contents
- Catalog Number 2025: IKK alpha Antibody
- Catalog Number 2121: IKK beta Antibody
- Catalog Number 2335: IKK gamma Antibody
- Catalog Number 2329: IKK epsilon Antibody
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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