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Home / Immunoblots / Human Lung Tumor Blot V

Human Lung Tumor Blot V

CATALOG NUMBER: 1514
  • Tested Applications: WB
  • Size: One blot

Specifications

  • SPECIES: Human
  • SAMPLES LOADED: Lanes: 1, protein markers; 2, A549; 3, small cell carcinoma; 4, matched normal lung tissue; 5, differentiated carcinoma; 6, matched normal lung tissue; 7, pleomorphic carcinoma; and 8, matched normal lung tissue.
  • TESTED APPLICATIONS: WB
  • APPLICATION NOTE: For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.

Properties

  • GEL PERCENTAGE: Gradient 4-20%
  • STORAGE CONDITIONS: Store at 4°C. The blot can be reused after stripping.

Additional Info

  • USER NOTE: Note: Lysate lane order may vary; please consult accompanying shipment paperwork.

Background

  • The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries the cell line A549 (human lung carcinoma), and 3 different human lung tumor tissue lysates and matched normal lysates (from non-tumor lung tissue from near the tumor) with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 differentiated carcinoma, and 1 pleomorphic carcinoma.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: This product is for research use only.

CATALOG NUMBER: 1514

  • Size: One blot
  • List Price: $598.00
Datasheet
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