- SPECIES: Human
- SAMPLES LOADED: Lanes: 1, protein markers; 2, A549; 3, small cell carcinoma; 4, matched normal lung tissue; 5, non-small cell carcinoma; 6, matched normal lung tissue; 7, squamous cell carcinoma; and 8, matched normal lung tissue.
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
- GEL PERCENTAGE: Gradient 4-20%
- STORAGE CONDITIONS: Store at 4°C. The blot can be reused after stripping.
- USER NOTE: Note: Lysate lane order may vary; please consult accompanying shipment paperwork.
- The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries the cell line A549 (human lung carcinoma), and 3 different human lung tumor tissue lysates and matched normal lysates (from non-tumor lung tissue from near the tumor) with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 non-small cell carcinoma, and 1 squamous cell carcinoma.
- FOR RESEARCH USE ONLY
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- Disclaimer: This product is for research use only.
CATALOG NUMBER: 1511
- Size: One blot
- List Price: $575.00
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