- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This HRH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human HRH3.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC-P, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
- PREDICTED MOLECULAR WEIGHT: 49 kDa
- PURIFICATION: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: HRH3
- ADDITIONAL NAMES: Histamine H3 receptor, H3R, HH3R, G-protein coupled receptor 97, HRH3, GPCR97
- Protein Accession Number: Q9Y5N1
- PROTEIN GI NUMBER: 17367264
- NCBI GENE ID NUMBER: 11255
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. HRH3 has significant sequence homology to members of the biogenic amine subfamily of GPCRs. Most notable is an aspartic acid residue in the predicted third transmembrane domain, which is a hallmark of the biogenic amine receptor subfamily; this residue is the putative binding site for the primary amine. HRH3 shares 22% and 21.4% amino acid sequence homology with the H1 and H2 receptors, respectively. Expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Northern blot analysis of human tissues showed HRH3 expression only in the brain, with highest expression in the thalamus and caudate nucleus. Whereas Northern blot analysis did not detect HRH3 expression in any peripheral tissue examined, RT-PCR showed expression in human small intestine, testis, and prostate. In situ hybridization of rat brain sections showed that Hrh3 is abundantly expressed in brain. Hrh3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle. In addition, Hrh3 expression was found in the locus ceruleus and in the histaminergic cell bodies in the tuberomammillary nuclei.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 58-874
- Size: 400 ul
- List Price: $445.00
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