- Trial Size
HOPX Antibody
CATALOG NUMBER: 8143- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (88%), Mouse: (88%), Bovine: (88%), Pig: (81%), Chicken: (81%)
- IMMUNOGEN: HOPX antibody was raised against a 16 amino acid peptide near the amino terminus of human HOPX.
The immunogen is located within the first 50 amino acids of HOPX. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: HOPX antibody can be used for detection of HOPX by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: HOPX antibody is human specific. At least three isoforms of HOPX are known to exist; this antibody will detect all three isoforms.
- POSITIVE CONTROL 1: Cat. No. 1303 - Human Brain Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-501 - Human Heart Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 12 kDa
Observed: 18 kDa
Properties
- PURIFICATION: HOPX antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: HOPX antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: HOPX antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: HOPX
- ADDITIONAL NAMES: HOP homeobox, homeodomain-only protein, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO
- Protein Accession Number: NP_001138932
- PROTEIN GI NUMBER: 224451025
- NCBI GENE ID NUMBER: 84525
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- HOPX is a small homeodomain protein that lacks normally conserved residues required for DNA binding (1). It is thought to act downstream of NKX2-5 and modulates serum response factor (SRF)-dependent cardiac-specific gene expression and cardiac development (1). HOPX also acts a tumor suppressor gene in multiple tumors and cancer cell lines (2,3). HOPX has been reported to regulate the proliferation/differentiation homeostasis in different cell types, including keratinocytes (4).
- 1: Chen F, Kook H, Milewski R, et al. Hop is an unusual homeobox gene that modulates cardiac development. Cell 2002; 110:713-23.
- 2: Asanoma K, Matsuda T, Kondo H, et al. NECC1, a candidate choriocarcinoma suppressor gene that encodes a homeodomain consensus motif. Genomics 2003; 81:15-25.
- 3: Katoh H, Yamashita K, Waraya M, et al. Epigenetic silencing of HOPX promotes cancer progression in colorectal cancer. Neoplasia 2012; 14:559-71.
- 4: Obarzanek-Fojt M, Favre B, Kypriotou M, et al. Homeodomain-only protein HOP is a novel modulator of late differentiation in keratinocytes. Eur. J. Cell Biol. 2011; 90:279-90.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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