- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human Histone H2AX (NP_002096.1).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, IHC, WB
- APPLICATION NOTE: WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
IF: 1:50 - 1:200
- POSITIVE CONTROL 1: HeLa
- POSITIVE CONTROL 2: MCF-7
- POSITIVE CONTROL 3: NIH/3T3
- POSITIVE CONTROL 4: Raji
- POSITIVE CONTROL 5: Mouse testis
- POSITIVE CONTROL 6: Mouse thymus
- PREDICTED MOLECULAR WEIGHT: Observed: 16kDa
- PURIFICATION: Affinity purification
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at -20°C. Avoid freeze / thaw cycles.
- NCBI OFFICIAL SYMBOL: H2AFX
- ADDITIONAL NAMES: H2AFX, H2A.X, H2A/X, H2AX
- NCBI GENE ID NUMBER: 3014
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 13-997
- Size: 50 uL
- List Price: $295.00
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