Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rb
- IMMUNOGEN: This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 amino acids from the N-terminal region of human HIST1H1B.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 23 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: HIST1H1B
- ADDITIONAL NAMES: Histone H15, Histone H1a, Histone H1b, Histone H1s-3, HIST1H1B, H1F5
- Protein Accession Number: P16401
- PROTEIN GI NUMBER: 19856407
- NCBI GENE ID NUMBER: 3009
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family.
- 1: Happel, N., et al. J. Mol. Biol. 386(2):339-350(2009)
- 2: Trojer, P., et al. Cell 129(5):915-928(2007)
- 3: Wisniewski, J.R., et al. Mol. Cell Proteomics 6(1):72-87(2007)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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