H3F3B Antibody
CATALOG NUMBER: 13-512- Clonality: Polyclonal
- Tested Applications: IF, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 50 uL
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human H3F3B (NP_002098.1).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, WB
- APPLICATION NOTE: WB: 1:500 - 1:2000
IF: 1:50 - 1:200 - POSITIVE CONTROL 1: A-549
- POSITIVE CONTROL 2: SW620
- POSITIVE CONTROL 3: U-87MG
- POSITIVE CONTROL 4: MCF7
- POSITIVE CONTROL 5: LO2
- PREDICTED MOLECULAR WEIGHT: Observed: 15kDa
Properties
- PURIFICATION: Affinity purification
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at -20°C. Avoid freeze / thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: H3F3B
- ADDITIONAL NAMES: H3.3B, histone H3.3, H3 histone, family 3B (H3.3B)
- NCBI GENE ID NUMBER: 3021
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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