H2AFX Antibody
CATALOG NUMBER: 57-219- Clonality: Polyclonal
- Tested Applications: IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This H2AFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human H2AFX.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC-P, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 15 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: H2AFX
- ADDITIONAL NAMES: Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX
- Protein Accession Number: P16104
- PROTEIN GI NUMBER: 121992
- NCBI GENE ID NUMBER: 3014
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Histones are basic nuclear proteins that are responsiblefor the nucleosome structure of the chromosomal fiber ineukaryotes. Two molecules of each of the four core histones (H2A,H2B, H3, and H4) form an octamer, around which approximately 146 bpof DNA is wrapped in repeating units, called nucleosomes. Thelinker histone, H1, interacts with linker DNA between nucleosomesand functions in the compaction of chromatin into higher orderstructures. This gene encodes a member of the histone H2A family,and generates two transcripts through the use of the conservedstem-loop termination motif, and the polyA addition motif.
- 1: Roch-Lefevre, S., et al. Radiat. Res. 174(2):185-194(2010)
- 2: Schmid, T.E., et al. Int. J. Radiat. Biol. 86(8):682-691(2010)
- 3: Jiang, X., et al. FEBS Lett. 584(13):2926-2930(2010)
- 4: Vasireddy, R.S., et al. Br. J. Cancer 102(10):1511-1518(2010)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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