Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Dog, Human, Mouse, Rat
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2B.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC, WB
- APPLICATION NOTE: GTF2B antibody can be used for detection of GTF2B by ELISA at 1:312500. GTF2B antibody can be used for detection of GTF2B by western blot at 4.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: Cat. No. 1211 - HepG2 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 35 kDa
Properties
- PURIFICATION: Antibody is purified by protein A chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store GTF2B antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: GTF2B
- ADDITIONAL NAMES: GTF2B, TF2B, TFIIB
- Protein Accession Number: NP_001505
- PROTEIN GI NUMBER: 4504193
- NCBI GENE ID NUMBER: 2959
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- GTF2B is the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.
- 1: Tubon, T.C., et al., (2004) Mol. Cell. Biol. 24 (7), 2863-2874.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
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