GRB2 Antibody
CATALOG NUMBER: 45-066- Clonality: Polyclonal
- Tested Applications: E, IHC, WB
- Host Species: Goat
- Species Reactivity: Human, Mouse, Pig, Rat
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human, Mouse, Pig, Rat
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog, Cow
- IMMUNOGEN: The immunogen for this antibody is: C-PRNYVTPVNRNV
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use at 0.05-0.15ug/ml.Immunohistochemistry:In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
- POSITIVE CONTROL 1: Cat. No. 1205 - Jurkat Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1315 - Human Tonsil Tissue Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: GRB2
- ADDITIONAL NAMES: GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, abundant SRC homology
- Protein Accession Number: NP_002077.1,NP_987102.1
- PROTEIN GI NUMBER: 4504111
- NCBI GENE ID NUMBER: 2885
Background
- 1: Lowenstein EJ, Daly RJ, Batzer AG, Li W, Margolis B, Lammers R, Ullrich A, Skolnik EY, Bar-Sagi D, Schlessinger J. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. Cell. 1992 Aug 7;70(3):431-42.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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