Close Hamburger Menu Close Hamburger Menu
Home / Apoptosis / Granzyme B Antibody [B18.1]
Granzyme B Antibody [B18.1]
Granzyme B Antibody [B18.1]

Granzyme B Antibody [B18.1]

CATALOG NUMBER: XA-1005
  • Clonality: Monoclonal
  • Tested Applications: Flow, ICC, IHC, IP, WB
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 0.1 mg

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Granzyme B (monoclonal B18.1) antibody was raised against recombinant human granzyme B, amino acids 7-227.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, ICC, IHC, IP, WB
  • APPLICATION NOTE: Granzyme B antibody can be used for detection of human granzyme B by flow cytometry, immunocytochemistry, immunohistochemistry (frozen sections, paraffin sections), immunoprecipitation and western blot. Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg of FITC- labeled B18.1 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 μg/mL of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Method: : Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

Properties

  • PURIFICATION: Granzyme B (monoclonal B18.1) antibody was purified to ≥95% in SDS-PAGE.
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG2a
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS containing 0.02% sodium azide
  • CONCENTRATION: batch dependent
  • STORAGE CONDITIONS: Granzyme B antibody can be stored at 4°C, stable for one year.

Additional Info

  • NCBI GENE ID NUMBER: 3002
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • 1: Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation: S. Legros-Maida, et al.; Eur. J. Immunol. 24, 229 (1994).
  • 2: Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients: M.V. Clement, et al.; Transplantation 57, 322 (1994).
  • 3: Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor: C. Berthou, et al.; Blood 86, 3500 (1995).
  • 4: Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long- term surviving lung transplanted patients: C. Berthou, et al.; Transplant. Proc. 28, 1964 (1996).

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: XA-1005

  • Size: 0.1 mg
  • List Price: $420.00
Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services 

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products
HIV-1 p24 Antibody [10A2] (biotin)
HIV-1 p24 Antibody [10A2] (biotin)

CATALOG NUMBER: PM-7371-biotin

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [8D7E11] (biotin)
HIV-1 p24 Antibody [8D7E11] (biotin)

CATALOG NUMBER: PM-7367-biotin