![Granulocyte Antibody [SPM250]](https://www.prosci-inc.com/wp-content/uploads/2021/11/Granulocyte-Antibody-SPM250_IHC_34-205_1.jpg){kind=small}
Specifications
- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human, Macaque
- IMMUNOGEN: Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this Granulocyte antibody.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IF, IHC
- APPLICATION NOTE: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the Granulocyte antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Properties
- PURIFICATION: Protein G affinity chromatography
- CLONALITY: Monoclonal
- ISOTYPE: IgG1
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.2 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Additional Info
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
Background
- Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. SPM250 Ab reacts with early precursor and mature forms of human myeloid cells. This mAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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