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Home / Flow / Glycophorin A Antibody [GYPA/280]
Glycophorin A Antibody [GYPA/280]Glycophorin A Antibody [GYPA/280]Glycophorin A Antibody [GYPA/280]
Glycophorin A Antibody [GYPA/280]
Glycophorin A Antibody [GYPA/280]
Glycophorin A Antibody [GYPA/280]

Glycophorin A Antibody [GYPA/280]

CATALOG NUMBER: 33-385
  • Clonality: Monoclonal
  • Tested Applications: Flow, IF, IHC-P, WB
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Recombinant human GPA protein was used as the immunogen for the Glycophorin A antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, IF, IHC-P, WB
  • APPLICATION NOTE: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
    Immunofluorescence: 0.5-1 ug/ml
    Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
    Prediluted format : incubate for 30 min at RT (2)
    Optimal dilution of the Glycophorin A antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: GYPA
  • ADDITIONAL NAMES: GYPA, glycophorin A (includes MN blood group), HGNC:4702, GPA, GPErik, GPSAT, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS, Mi.V glycoprotein (24 AA), erythroid-lineage-specific membrane sialoglycoprotein, glycophorin A, glycophorin Er
  • NCBI GENE ID NUMBER: 2993
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-385

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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