- Trial Size
GLIS2 Antibody
CATALOG NUMBER: 8091- Clonality: Polyclonal
- Tested Applications: E, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: GLIS2 antibody was raised against an 18 amino acid peptide near the center of human GLIS2.
The immunogen is located within amino acids 260 - 310 of GLIS2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC-P, WB
- APPLICATION NOTE: GLIS2 antibody can be used for detection of GLIS2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. - SPECIFICITY: GLIS2 antibody is human, mouse and rat reactive.
- POSITIVE CONTROL 1: Cat. No. 1204 - K562 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 58 kDa
Observed: 60 kDa
Properties
- PURIFICATION: GLIS2 antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: GLIS2 antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: GLIS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: GLIS2
- ADDITIONAL NAMES: GLIS family zinc finger 2, GLI-similar 2, neuronal krueppel-like protein, NKL, NPHP7
- Protein Accession Number: NP_115964
- PROTEIN GI NUMBER: 110431364
- NCBI GENE ID NUMBER: 84662
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- GLIS2, also known as neuronal Krueppel-like, is a 524 amino acid protein that belongs to the GLI C2H2-type zinc-finger protein family (1). GLIS2 can act either as a transcription repressor or as a transcription activator and may be involved in neuron differentiation (1,2). GLIS2 is expressed at high levels in kidney and at low levels in heart, lung and placenta. Mutations of GLIS2 have been suggested to be associated with development of progressive chronic kidney disease with characteristics resembling nephronophthisis (3,4).
- 1: Vasanth S, ZeRuth G, Kang HS, et al. Identification of nuclear localization, DNA binding, and transactivating mechanisms of Kruppel-like zinc finger protein Gli-similar 2 (Glis2). J. Biol. Chem. 2011; 286:4749-59.
- 2: Hosking CR, Ulloa F, Hogan C, et al. The transcriptional repressor Glis2 is a novel binding partner for p120 catenin. Mol. Biol. Cell 2007; 18:1918-27.
- 3: Kim YS, Kang HS, Herbert R, et al. Kruppel-like zinc finger protein Glis2 is essential for the maintenance of normal renal functions. Mol. Cell Biol. 2008; 28:2358-67.
- 4: Attanasio M, Uhlenhaut NH, Sousa VH, et al. Loss of GLIS2 causes nephronophthisis in humans and mice by increased apoptosis and fibrosis. Nat. Genet. 2007; 39:1018-24.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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