- Trial Size
GFR alpha 1 Antibody
CATALOG NUMBER: 1133- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: GFR alpha 1 antibody was raised against a 14 amino acid peptide near the center of human GFR alpha 1.
The immunogen is located within amino acids 360 - 410 of GFR alpha 1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: GFR alpha 1 antibody can be used for detection of GFR alpha 1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: GFR alpha 1 antibody is predicted to not cross-react with other members of the GFR alpha family of proteins.
- POSITIVE CONTROL 1: Cat. No. 1303 - Human Brain Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1304 - Human Liver Tissue Lysate
- POSITIVE CONTROL 3: Cat. No. 1305 - Human Kidney Tissue Lysate
- POSITIVE CONTROL 4: Cat. No. 1306 - Human Spleen Tissue Lysate
- POSITIVE CONTROL 5: Cat. No. 10-301 - Human Brain Tissue Slide
- PREDICTED MOLECULAR WEIGHT: 51 kDa
Properties
- PURIFICATION: GFR alpha 1 Antibody is Antibody is DEAE purified.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: GFR alpha 1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: GFR alpha 1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: GFRA1
- ADDITIONAL NAMES: GFR alpha 1 Antibody: GDNFR, RET1L, RETL1, TRNR1, GDNFRA, GFR-ALPHA-1, GDNF family receptor alpha-1, RET ligand 1, GDNF receptor alpha-1
- Protein Accession Number: P56159
- PROTEIN GI NUMBER: 20141405
- NCBI GENE ID NUMBER: 2674
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- GFR alpha 1 Antibody: Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRα-1 recently. GFRα-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRα and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.
- 1: Jing S, Wen D, Yu Y, et al. GDNF-induced activation of the Ret protein tyrosine kinase is mediated by GDNFR-a, a novel receptor for GDNF. Cell 1996; 85:1113-24.
- 2: Treanor JJS, Goodman L, Sauvage FD, et al. Characterization of a multicomponent receptor for GDNF. Nature 1996;82:80-83.
- 3: Sanicola M, Hession C, Worley D, et al. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc. Natl. Acad. Sci. USA 1997; 94:6238-43.
- 4: Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997; 387:721-4
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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