- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Rabbit polyclonal FOXP3 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human FOXP3.
The immunogen is located within the last 50 amino acids of FOXP3.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: FOXP3 antibody can be used for detection of FOXP3 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- POSITIVE CONTROL 1: Cat. No. 1203 - A549 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 47 kDa
- PURIFICATION: FOXP3 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: FOXP3 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: FOXP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
- NCBI OFFICIAL SYMBOL: FOXP3
- ADDITIONAL NAMES: FOXP3 Antibody: JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2, Forkhead box protein P3, Scurfin
- Protein Accession Number: NP_054728
- PROTEIN GI NUMBER: 31982943
- NCBI GENE ID NUMBER: 50943
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- FOXP3 Antibody: FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. FOXP3 acts as a repressor of transcription and regulates T cell activation, with its overexpression in CD4 T cells leading to an attenuation of activation-induced cytokine production and proliferation. In regulatory T (Treg) cells, FOXP3 is essential for Treg suppressor function and its expression leads to the repression of IL-17 expression. Genetic mutations involving FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome.
- 1: Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat. Genet. 2001; 27:68-73
- 2: Schubert LA, Jeffery E, Zhang Y, et al. Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation. J. Biol. Chem. 2001; 276:37672-9.
- 3: Gavin MA, Rasmussen JP, Fontenot JD, et al. Foxp3-dependent programme of regulatory T-cell differentiation. Nature 2007; 445:771-5
- 4: Kobayashi I, Shiari R, Yamada M, et al. Novel mutations of FOXP3 in two Japanese patients with immune dysregulation, polyendocrinopathy, enteropathy, X linked syndrome (IPEX). J. Med. Genet. 2001; 38:874-6.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 7187
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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