- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Drosophila, Human, Rat
- IMMUNOGEN: Yeast nuclear preparations.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC, WB
- SPECIFICITY: Specific for the ~34kDa Fibrillarin /Nop1p protein.
- PREDICTED MOLECULAR WEIGHT: 34
- PURIFICATION: Total IgG fraction
- CLONALITY: Monoclonal
- ISOTYPE: IgG1
- PHYSICAL STATE: Liquid
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Fibrillarin antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
- NCBI OFFICIAL SYMBOL: NOP1
- Protein Accession Number: P15646
- PROTEIN GI NUMBER: 119857
- NCBI GENE ID NUMBER: 851548
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
- 1: Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol Cell 54:123-133 (1985).
- 2: Aris JP and Blobel G. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J. Cell Biol. 107:17-31 (1988).
- 3: Newton K, Petfalski E, Tollervey D, Caceres JF. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol. 23:8519-8527 (2003).
- FOR RESEARCH USE ONLY
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CATALOG NUMBER: 50-266
- Size: 0.1 mL
- List Price: $430.00
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