- Trial Size
FHIT Antibody
CATALOG NUMBER: 7455- Clonality: Polyclonal
- Tested Applications: E, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (88%), Rat: (72%)
- IMMUNOGEN: FHIT antibody was raised against an 18 amino acid peptide near the carboxy terminus of human FHIT.
The immunogen is located within the last 50 amino acids of FHIT . - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: FHIT antibody can be used for detection of FHIT by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: FHIT antibody is human and mouse reactive.
- POSITIVE CONTROL 1: Cat. No. 1201 - HeLa Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-001 - HeLa Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 16 kDa
Observed: 15kDa
Properties
- PURIFICATION: FHIT Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: FHIT Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: FHIT antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: FHIT
- ADDITIONAL NAMES: FHIT Antibody : FRA3B, AP3AaseAP3A hydrolase, AP3Aase
- Protein Accession Number: NP_002003
- PROTEIN GI NUMBER: 4503719
- NCBI GENE ID NUMBER: 2272
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- FHIT is member of the histidine triad gene family and is a diadenosine involved in purine metabolism (1). FHIT is also thought to be a tumor suppressor gene and is involved in multiple apoptotic pathways (1,2). The FHIT gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene (3). Aberrant transcripts from this gene have been found in multiple carcinomas (4).
- 1: Barnes LD, Garrison PN, Siprashvili Z, et al. Fhit, a putative tumor suppressor in humans, is a dinucleotide 5',5'''-P1,P3-triphosphate hydrolase. Biochemistry 1996; 35:11529-35.
- 2: Wali A. FHIT: doubts are clear now. ScientificWorldJournal 2010; 10:1142-51.
- 3: Ohta M, Inoue H, Cotticelli MG, et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell 1996; 84:587-97.
- 4: Drusco A, Pekarsky Y, Costinean S, et al. Common fragile site tumor suppressor genes and corresponding mouse models of cancer. J. Biomed. Biotechnol.2011; Epub 2010 Dec 29.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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