Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-4. Human FGF-4 specific antibody was purified by affinity chromatography employing immobilized hFGF-4 matrix.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: ELISA:
Indirect:
To detect hFGF-4 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-4.
Sandwich
To detect hFGF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human FGF-4 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-4.
Western Blot:
To detect hFGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: FGF-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: FGF4
- ADDITIONAL NAMES: HST, KFGF, HST-1, HSTF1, K-FGF, HBGF-4, HST, KS3, Fibroblast growth factor 4, Heparin secretory-transforming protein 1, FGF-4
- Protein Accession Number: P08620
- PROTEIN GI NUMBER: 122750
- NCBI GENE ID NUMBER: 2249
- USER NOTE: Centrifuge vial prior to opening.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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