Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Drosophila, Human, Mouse
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBXW7.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: FBXW7 antibody can be used for detection of FBXW7 by ELISA at 1:312500. FBXW7 antibody can be used for detection of FBXW7 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: Cat. No. 1205 - Jurkat Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 66 kDa
Properties
- PURIFICATION: Antibody is purified by peptide affinity chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store FBXW7 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: FBXW7
- ADDITIONAL NAMES: FBXW7, AGO, CDC4, DKFZp686F23254, FBW7, FBX30, FBXW6, SEL-10, SEL10, FBW6, hAgo, hCdc4, FBXO30
- Protein Accession Number: NP_001013433
- PROTEIN GI NUMBER: 61743926
- NCBI GENE ID NUMBER: 55294
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- FBXW7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in FBXW7 gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene.
- 1: Lee, J.W., Pathology 40 (3), 305 (2008).
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.