FasL Antibody [Mike-2]
CATALOG NUMBER: XA-1003- Clonality: Monoclonal
- Tested Applications: Flow, WB
- Host Species: Rat
- Species Reactivity: Human
- Conjugate: Unconjugated
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- HOST SPECIES: Rat
- SPECIES REACTIVITY: Human
- IMMUNOGEN: FasL (monoclonal Mike-1) antibody was raised against a recombinant human soluble FasL from amino acids 103-281.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, WB
- APPLICATION NOTE: FasL antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: Cell extracts from FasL transfected cells (5x104) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL Mike-2 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system
Properties
- PURIFICATION: FasL (monoclonal Mike-1) antibody was purified to ≥95% in SDS-PAGE from concentrated hybridoma culture supernatant.
- CLONALITY: Monoclonal
- ISOTYPE: IgG2a
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: FasL antibody can be stored at 4°C, stable for one year.
Additional Info
- NCBI GENE ID NUMBER: 356
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- 1: Constitutive Expression of FasL in Thyrocytes: T.A. Stokes, et al.; Science 279, 2015a (1998): T.A. Stokes, et al.; Science 279, 2015a (1998), Technical comments.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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