- Trial Size
EVER2 Antibody
CATALOG NUMBER: 4605- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
Powered by BiozSpecifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: EVER2 antibody was raised against a 14 amino acid synthetic peptide from near the center of human EVER2.
The immunogen is located within amino acids 300 - 350 of EVER2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: EVER2 antibody can be used for detection of EVER2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: At least three isoforms of EVER2 are known to exist; this antibody will only recognize the larger isoform. EVER2 has no cross-reactivity to EVER1.
- POSITIVE CONTROL 1: Cat. No. 1205 - Jurkat Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 10-301 - Human Brain Tissue Slide
Properties
- PURIFICATION: EVER2 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: EVER2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: EVER2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: TMC8
- ADDITIONAL NAMES: EVER2 Antibody: EV2, EVER2, EVIN2, Transmembrane channel-like protein 8, Epidermodysplasia verruciformis protein 2
- Protein Accession Number: AAM44454
- PROTEIN GI NUMBER: 25527192
- NCBI GENE ID NUMBER: 147138
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- EVER2 Antibody: Epidermodysplasia verruciformis (EV) is an autosomal recessive genodermatosis associated with a high risk of skin cancers resulting from a high susceptibility to infection by specific human papillomaviruses. Mutations in two homologous genes EVER1 and EVER2 cause the majority of EV cases. These two proteins form a complex and interact with the zinc transporter ZnT-1 in the endoplasmic reticulum. Cells lacking EVER2 accumulated higher levels of zinc in the nucleolus and nucleus compare to those cells with and intact EVER2 gene, indicating that one role of EVER2 is to regulate the intracellular distribution of zinc.
- 1: Ramoz N, Taieb A, Rueda L-A, et al. Evidence for a nonallelic heterogeneity of epidermodysplasia verruciformis with two susceptibility loci mapped to chromosome regions 2p21-p24 and 17q25. J. Invest. Dermatol.2000; 114:1148-53.
- 2: Ramoz N, Rueda L-A, Bouadjar B, et al. Mutations in two adjacent novel genes are associated with epidermodysplasia verruciformis. Nat. Genet.2002; 32:579-81.
- 3: Lazarczyk M, Pons C, Mendoza J-A, et al. Regulation of cellular zinc balance as a potential mechanism of EVER-mediated protection against pathogenesis by cutaneous oncogenic human papillomaviruses. J. Exp. Med.2008; 205:35-42.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
New & Featured Products
![HIV-1 p24 Antibody [10A2] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-10A2_WB_PM-7371.gif){kind=small}
HIV-1 p24 Antibody [10A2] (biotin)
CATALOG NUMBER: PM-7371-biotin
![HIV-1 p24 Antibody [9D2F6] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-9D2F6_WB_PM-7369.gif){kind=small}
HIV-1 p24 Antibody [9D2F6] (biotin)
CATALOG NUMBER: PM-7369-biotin
![HIV-1 p24 Antibody [8D7E11] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-8D7E11_WB_PM-7367.gif){kind=small}
HIV-1 p24 Antibody [8D7E11] (biotin)
CATALOG NUMBER: PM-7367-biotin