- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Horse, Human, Monkey, Mouse, Pig, Rat, Sheep
- IMMUNOGEN: The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IF, IHC, WB
- APPLICATION NOTE: Flow Cytometry: 0.5-1ug/10e6 cells
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
- PURIFICATION: Protein G purified monoclonal antibody
- CLONALITY: Monoclonal
- ISOTYPE: IgG2a
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.2 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
- NCBI OFFICIAL SYMBOL: ESR2
- ADDITIONAL NAMES: Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2
- NCBI GENE ID NUMBER: 2100
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
- Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including alpha and beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER alpha and beta are differentially activated by various ligands. Ligand interaction triggers a cascade of events, including dissociation from heat shock proteins, recepter dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and beta may be regulated by distinct mechanisms even though they share many functional characteristics.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 33-129
- Size: 100 ug
- List Price: $535.00
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