ERK2 Antibody
CATALOG NUMBER: 45-551- Clonality: Polyclonal
- Tested Applications: ELISA, IF, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
- IMMUNOGEN: The immunogen for this antibody is: CAAGPEMVRGQVF
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10ug/ml.
- SPECIFICITY: This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
- POSITIVE CONTROL 1: Cat. No. 1211 - HepG2 Cell Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: MAPK1
- ADDITIONAL NAMES: MAPK1, ERK2, ERK, p38, p40, p41, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2, mitogen-activated protein kinase 2, extracellular signal-regulated kinase 2, extracellular signal-regulated kinase-2
- Protein Accession Number: NP_002736.3,NP_620407.1
- PROTEIN GI NUMBER: 66932916
- NCBI GENE ID NUMBER: 5594
Background
- 1: Lee SM, Nguyen TH, Park MH, Kim KS, Cho KJ, Moon DC, Kim HY, Yoon do Y, Hong JT. EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes. Biochem Biophys Res Commun. 2004 Aug 6;320(4):1087-95.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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