ER Signaling Protein Detection Set
CATALOG NUMBER: PSI-1814- Clonality: Polyclonal
- Tested Applications: IF, IHC, WB
- Species Reactivity: Human
- Size: 1 Set
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- SPECIES REACTIVITY: Human
- IMMUNOGEN: Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
- TESTED APPLICATIONS: IF, IHC, WB
- SET CONTENTS: ATF Antibody (CT), Catalog No. 3681 (50 μg)
Caspase 12 Antibody (lrg), Catalog No. 3195 (50 μg)
IRE1p Antibody (CT), Catalog No. 3655 (50 μg)
XBP1 Antibody (CT), Catalog No. 3685 (50 μg) - APPLICATION NOTE: These polyclonal antibodies can be used for detection of ATF6, Caspase 12, IRE1p and XBP1 by immunoblot at 1 - 2 μg/mL. ATF6, Caspase 12, IRE1p and XBP1 antibodies can detect their respective proteins via immunohistochemistry at 1 - 10 μg/mL, and Immunoflourescence.
- POSITIVE CONTROL 1: ATF6 antibody: MCF7 cell lysate, Catalog No. 1219
Caspase 12 antibody: Human heart tissue lysate, Catalog No. 1301
IRE1p antibody: A-20 cell lysate, Catalog No. 1288
XBP1 antibody: PC-3 cell lysate, Catalog No. 1216
Properties
- PURIFICATION: Antibodies are supplied as affinity chromatography purified IgG.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide.
- CONCENTRATION: Antibody 1 mg/mL
- STORAGE CONDITIONS: Stable at 4°C for three months, store at -20°C for up to one year.
Additional Info
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The endoplasmic reticulum (ER) is an organelle composed of an interconnected network of tubules, vesicles and cisternae found in all eukaryotic cells. It is involved in several specialized processes such as protein translation, folding, and transport of proteins to be used in the cell membrane or secreted from the cell. Accumulation of malfolded proteins in the ER activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase, to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA, converting it to a potent UPR transcriptional activator. ER stress also causes the cleavage of ATF6, yielding a cytosolic fragment which migrates to the nucleus and together with XBP-1, activates transcription of UPR-responsive genes. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.
For images please see PDF data sheet - 1: Kleizen B and Braakman I. Protein folding and quality control in the endoplasmic reticulum. Curr. Opin. Cell Biol. 2004; 16:343-9.
- 2: Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.
- 3: Lee AS. The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress. Methods 2005; 35:373-81.
- 4: Bertolotti A, Zhang Y, Hendershot LM, et al. Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response. Nat. Cell Biol. 2000; 2:326-32.
Set Contents
- Catalog Number 3681: ATF6 Antibody
- Catalog Number 3195: Caspase-12 Antibody (Large)
- Catalog Number 3655: IRE1p Antibody
- Catalog Number 3685: XBP-1 Antibody
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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