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Home / Flow / Epithelial Marker Antigen Antibody [MUC1/967]
Epithelial Marker Antigen Antibody [MUC1/967]Epithelial Marker Antigen Antibody [MUC1/967]Epithelial Marker Antigen Antibody [MUC1/967]
Epithelial Marker Antigen Antibody [MUC1/967]
Epithelial Marker Antigen Antibody [MUC1/967]
Epithelial Marker Antigen Antibody [MUC1/967]

Epithelial Marker Antigen Antibody [MUC1/967]

CATALOG NUMBER: 33-265
  • Clonality: Monoclonal
  • Tested Applications: Flow, IF, IHC
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Human milk fat globule membranes were used as the immunogen for this Epithelial Marker Antigen antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, IF, IHC
  • APPLICATION NOTE: Flow Cytometry: 0.5-1 ug/million cells
    IF: 1-2 ug/ml
    IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
    Prediluted format : incubate for 30 min at RT (2)
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Epithelial Marker Antigen antibody to be titered up or down for optimal performance.

    1. FFPE staining REQUIRES boiling sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 10-20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: MUC1
  • ADDITIONAL NAMES: MUC1 Antibody : EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC, Mucin-1, Breast carcinoma-associated antigen DF3
  • NCBI GENE ID NUMBER: 4582
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • This Epithelial Membrane Antigen / EMA antibody, also called MUC1 and Mucin-1, recognizes the full-length protein in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this mAb is APDTR in the VNTR region. It reacts with the core peptide of the EMA protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the mAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This EMA antibody reacts with both normal and malignant epithelia of various tissues including breast and colon.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-265

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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