Eotaxin (CCL11) Antibody
CATALOG NUMBER: 38-332- Clonality: Polyclonal
- Tested Applications: E, WB
- Host Species: Rabbit
- Species Reactivity: Mouse
- Conjugate: Unconjugated
- Size: 0.05 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Mouse
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mEotaxin. Anti-Murine Eotaxin specific antibody was purified by affinity chromatography employing immobilized mEotaxin matrix.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: Sandwich ELISA: To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine Eotaxin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin.
Western Blot
To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Eotaxin specific antibody was purified by affinity chromatography employing immobilized mEotaxin matrix
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: Ccl11
- ADDITIONAL NAMES: CCL11
- Protein Accession Number: P48298
- PROTEIN GI NUMBER: P48298.1
- NCBI GENE ID NUMBER: 20292
- USER NOTE: Centrifuge vial prior to opening.
Background
- Eotaxin is a CC chemokine that signals through the CCR3 receptor. It is produced by IFN-gamma-stimulated endothelial cells and TNF-activated monocytes. Eotaxin selectively chemoattracts eosinophils and, along with Eotaxin-2 and Eotaxin-3, plays a key role in the regulation of eosinophil recruitment in the asthmatic lung and in allergic reactions. Recombinant Murine Eotaxin is an 8.4 kDa protein containing 74 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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