- Trial Size
Eotaxin Antibody
CATALOG NUMBER: 1123- Clonality: Polyclonal
- Tested Applications: E, ICC, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (80%)
- IMMUNOGEN: Eotaxin antibody was raised in rabbits against a peptide corresponding to amino acids near the carboxy terminus of human eotaxin.
The immunogen is located within the last 50 amino acids of Eotaxin. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, ICC, IF, WB
- APPLICATION NOTE: Eotaxin antibody can be used for detection of eotaxin by Western blot at 1 μg/mL. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1201 - HeLa Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-010 - HEK293 Cell Slide
- PREDICTED MOLECULAR WEIGHT: 9 - 18 kDa
Properties
- PURIFICATION: Eotaxin Antibody is Antibody is DEAE purified.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: Eotaxin Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/ml
- STORAGE CONDITIONS: Eotaxin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: CCL11
- ADDITIONAL NAMES: Eotaxin Antibody: SCYA11, SCYA11, Eotaxin, C-C motif chemokine 11
- Protein Accession Number: CAB07027
- PROTEIN GI NUMBER: 2462478
- NCBI GENE ID NUMBER: 6356
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Eotaxin Antibody: Chemokines play a key role in inflammation. The CC chemokine eotaxin is a potent and specific eosinophil chemoattractant that is expressed by a variety of cell types in certain inflammatory conditions. Some G-protein coupled chemokine receptors are also utilized as virus coreceptors for fusion and infection of cells. The eotaxin receptor CCR3 is required for HIV-1 entry into target cells such as microglia and eotaxin inhibits the infection of HIV-1.
- 1: Kitaura M, Nakajima T, Imai T, et al. Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J Biol Chem 1996;271:7725-30
- 2: Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J Clin Invest 1996;97:604-12
- 3: He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 1997;385(6617):645-9
- 4: Choe H, Farzan M, Sun Y, et al. The β-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 1996;85:1135-48 (WD0500)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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